This Article Full Text Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Email this article to a colleague Similar articles in this journal Similar articles in Web of Science Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Request Permissions Citing Articles Citing Articles via HighWire Citing Articles via Web of Science (9) Citing Articles via Google Scholar Google Scholar Articles by Lan, W. Articles by Redmond, P. H. Search for Related Content PubMed PubMed Citation Articles by Lan, W. Articles by Redmond, P. H.

---

ANESTHETIC PHARMACOLOGY

Activated Endothelial Interleukin-1ß, -6, and -8 Concentrations and Intercellular Adhesion Molecule-1 Expression Are Attenuated by Lidocaine

Wei Lan, MB^*, Dominic C. Harmon, MD, FCARCSI, Jiang H. Wang, PhD^*, George D. Shorten, MD, PhD, FFARCSI, and Paul H. Redmond, FRCSI^*

*Academic Department of Surgery and Department of Anaesthesia and Intensive Care Medicine, Cork University Hospital, University College Cork, Ireland

Address correspondence and reprint requests to Wei Lan, MB, Academic Department of Surgery, Cork University Hospital, Cork, Ireland. Address e-mail to dweilan{at}hotmail.com.

Endothelial cells play a key role in ischemia reperfusion injury. We investigated the effects of lidocaine on activated human umbilical vein endothelial cell (HUVEC) interleukin (IL)-1ß, IL-6, and IL-8 concentrations and intercellular adhesion molecule-1 (ICAM-1) expression. HUVECs were pretreated with different concentrations of lidocaine (0 to 0.5 mg/mL) for 60 min, thereafter tumor necrosis factor- was added at a concentration of 2.5 ng/mL and the cells incubated for 4 h. Supernatants were harvested, and cytokine concentrations were analyzed by enzyme-linked immunosorbent assay. Endothelial ICAM-1 expression was analyzed by using flow cytometry. Differences were assessed using analysis of variance and post hoc unpaired Student’s t-test where appropriate. Lidocaine (0.5 mg/mL) decreased IL-1ß (1.89 ± 0.11 versus 4.16 ± 1.27 pg/mL; P = 0.009), IL-6 (65.5 ± 5.14 versus 162 ± 11.5 pg/mL; P < 0.001), and IL-8 (3869 ± 785 versus 14,961 ± 406 pg/mL; P < 0.001) concentrations compared with the control. IL-1ß, IL-6, and IL-8 concentrations in HUVECs treated with clinically relevant plasma concentrations of lidocaine (0.005 mg/mL) were similar to control. ICAM-1 expression on lidocaine-treated (0.05 mg/mL) HUVECs was less than on controls (198 ± 52.7 versus 298 ± 50.3; Mean Channel Fluorescence; P < 0.001). Activated endothelial IL-1ß, IL-6, and IL-8 concentrations and ICAM-1 expression are attenuated only by lidocaine at concentrations larger than clinically relevant concentrations.

This article has been cited by other articles:

				D. O. Zamora and J. W. Kiel Topical Proparacaine and Episcleral Venous Pressure in the Rabbit Invest. Ophthalmol. Vis. Sci., June 1, 2009; 50(6): 2949 - 2952. [Abstract] [Full Text] [PDF]

				B. N. Y. Setty and S. G. Betal Microvascular endothelial cells express a phosphatidylserine receptor: a functionally active receptor for phosphatidylserine-positive erythrocytes Blood, January 15, 2008; 111(2): 905 - 914. [Abstract] [Full Text] [PDF]

				J. G. Bovill Anesthetic Pharmacology: Reflections of a Section Editor Anesth. Analg., November 1, 2007; 105(5): 1186 - 1190. [Full Text] [PDF]