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ANESTHETIC PHARMACOLOGY

Activated Endothelial Interleukin-1ß, -6, and -8 Concentrations and Intercellular Adhesion Molecule-1 Expression Are Attenuated by Lidocaine

Wei Lan, MB^*, Dominic C. Harmon, MD, FCARCSI, Jiang H. Wang, PhD^*, George D. Shorten, MD, PhD, FFARCSI, and Paul H. Redmond, FRCSI^*

*Academic Department of Surgery and Department of Anaesthesia and Intensive Care Medicine, Cork University Hospital, University College Cork, Ireland

Address correspondence and reprint requests to Wei Lan, MB, Academic Department of Surgery, Cork University Hospital, Cork, Ireland. Address e-mail to dweilan{at}hotmail.com.

Endothelial cells play a key role in ischemia reperfusion injury. We investigated the effects of lidocaine on activated human umbilical vein endothelial cell (HUVEC) interleukin (IL)-1ß, IL-6, and IL-8 concentrations and intercellular adhesion molecule-1 (ICAM-1) expression. HUVECs were pretreated with different concentrations of lidocaine (0 to 0.5 mg/mL) for 60 min, thereafter tumor necrosis factor- was added at a concentration of 2.5 ng/mL and the cells incubated for 4 h. Supernatants were harvested, and cytokine concentrations were analyzed by enzyme-linked immunosorbent assay. Endothelial ICAM-1 expression was analyzed by using flow cytometry. Differences were assessed using analysis of variance and post hoc unpaired Student’s t-test where appropriate. Lidocaine (0.5 mg/mL) decreased IL-1ß (1.89 ± 0.11 versus 4.16 ± 1.27 pg/mL; P = 0.009), IL-6 (65.5 ± 5.14 versus 162 ± 11.5 pg/mL; P < 0.001), and IL-8 (3869 ± 785 versus 14,961 ± 406 pg/mL; P < 0.001) concentrations compared with the control. IL-1ß, IL-6, and IL-8 concentrations in HUVECs treated with clinically relevant plasma concentrations of lidocaine (0.005 mg/mL) were similar to control. ICAM-1 expression on lidocaine-treated (0.05 mg/mL) HUVECs was less than on controls (198 ± 52.7 versus 298 ± 50.3; Mean Channel Fluorescence; P < 0.001). Activated endothelial IL-1ß, IL-6, and IL-8 concentrations and ICAM-1 expression are attenuated only by lidocaine at concentrations larger than clinically relevant concentrations.

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Anesthesia & Analgesia® is published for the International Anesthesia Research Society® by Lippincott Williams & Wilkins with the assistance of Stanford University Libraries' HighWire Press®. Copyright 2006 by the International Anesthesia Research Society. Online ISSN: 1526-7598   Print ISSN: 0003-2999