This Article Full Text Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Email this article to a colleague Similar articles in this journal Similar articles in Web of Science Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Request Permissions Citing Articles Citing Articles via HighWire Citing Articles via Web of Science (5) Citing Articles via Google Scholar Google Scholar Articles by Torres Filho, I. P. Articles by Pittman, R. N. Search for Related Content PubMed PubMed Citation Articles by Torres Filho, I. P. Articles by Pittman, R. N. Related Collections Cardiovascular Blood

---

CARDIOVASCULAR ANESTHESIA

Systemic Responses to Hemodilution After Transfusion with Stored Blood and with a Hemoglobin-Based Oxygen Carrier

Ivo P. Torres Filho, MD, PhD^*, Bruce D. Spiess, MD^*, R. Wayne Barbee, PhD, Kevin R. Ward, MD, John Oldenhof, PhD, and Roland N. Pittman, PhD

Departments of *Anesthesiology, Emergency Medicine, and Physiology, Virginia Commonwealth University Reanimation Engineering Shock Center, Virginia Commonwealth University Medical Center, Richmond, Virginia; and Hemosol Inc., Toronto, Ontario, Canada

Address correspondence and reprint requests to Ivo P. Torres Filho, MD, PhD, Department of Anesthesiology, MCV-VCU, 1101 E. Marshall St., Room B1-012, Richmond, VA 23298. Address e-mail to itorres{at}vcu.edu.

We assessed the systemic effects of exchanges with blood or hemoglobin (Hb) raffimer under conditions of critical oxygen delivery (Do₂crit). We compared Do₂crit in animals receiving Hb-based oxygen carrier (HBOC; Hemolink^TM), fresh blood (collected <24 h), or stored blood (10 days) before hemodilution. Rats were randomized to control, blood, or HBOC isovolemic exchange. Oxygen consumption was measured by using expired gas (o₂a) and blood (o₂b) samples, whereas whole-body oxygen delivery (Do₂) was calculated from cardiac output and arterial oxygen content. After exchange, rats were subjected to stepwise isovolemic hemodilution. Blood pressure, gases, acid-base status, glucose, Hb oxygen saturation, heart rate, and total peripheral resistance were also measured. We found that 1) HBOC-treated rats showed an increased mean arterial blood pressure and total peripheral resistance throughout the hemodilution, 2) Do₂crit calculated with o₂a or o₂b gave identical results, 3) Do₂crit was not different between animals receiving blood and those receiving HBOC, 4) the terminal Hb concentration (1.8 ± 0.1 g/dL) and Do₂ (5 ± 1 mL · min^–1 · kg^–1) were similar for all animals, and 5) most oxygen transport and biochemical variables changed similarly during hemodilution. The data suggest that tolerance to Do₂crit is not altered by 50% replacement of native Hb by stored blood or Hb raffimer.

This article has been cited by other articles:

				A. Rigamonti, A. T. McLaren, C. D. Mazer, K. Nix, T. Ragoonanan, J. Freedman, A. Harrington, and G. M. T. Hare Storage of strain-specific rat blood limits cerebral tissue oxygen delivery during acute fluid resuscitation Br. J. Anaesth., March 1, 2008; 100(3): 357 - 364. [Abstract] [Full Text] [PDF]

				K. K. Tremper Hemoglobin-Based Oxygen Carrying Solutions: Will They Replace Red Blood Cells? Anesth. Analg., April 1, 2005; 100(4): 910 - 911. [Full Text] [PDF]