JOURNAL HOME CME HOME THIS MONTH PAST ISSUES ETOC COLLECTIONS AUTHORS REVIEWERS EDITORIAL BOARD FEEDBACK RSS HELP
		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Email this article to a colleague Similar articles in this journal Similar articles in ISI Web of Science Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via ISI Web of Science (4) Citing Articles via Google Scholar Google Scholar Articles by Nader, N. D. Articles by Knight, P. R. Search for Related Content PubMed PubMed Citation Articles by Nader, N. D. Articles by Knight, P. R.

---

CRITICAL CARE AND TRAUMA

Serine Antiproteinase Administration Preserves Innate Superoxide Dismutase Levels After Acid Aspiration and Hyperoxia but Does Not Decrease Lung Injury

Nader D. Nader, MD, PhD^*, Bruce A. Davidson, BS^*, Alan R. Tait, PhD, Bruce A. Holm, PhD, and Paul R. Knight, MD, PhD^*

Departments of *Anesthesiology, Microbiology, and Pharmacology, State University of New York at Buffalo; and Department of Anesthesiology, University of Michigan, Ann Arbor

Address correspondence and reprint requests to Nader D. Nader, MD, PhD, Associate Professor of Anesthesiology, Pathology, and Anatomical Sciences, SUNY at Buffalo, VA Western NY Healthcare System, 3495 Bailey Ave., Buffalo, NY 14215. Address e-mail to nnader{at}buffalo.edu.

Acute lung injury after acid aspiration and increased ambient oxygen result in significant oxidative damage to the lungs. Lung antioxidant levels are also reduced. Because levels of serine proteinases in the airspaces are also dramatically increased, we hypothesized that these enzymes play a role in degrading lung antioxidants. Rats were treated with a serine proteinase inhibitor, aprotinin, before pulmonary aspiration of acid in the presence of increased ambient oxygen (hyperoxia). Lung Cu/Zn and Mn superoxide dismutase (SOD) activity (by colorimetric assay) and Cu/Zn SOD immune reactive protein (enzyme-linked immunosorbent assay) were assayed. The effects of antiproteinase treatment on acute lung injury were also assessed. Total SOD, Cu/Zn SOD, and Cu/Zn SOD antigenic protein levels were decreased in animals after acid aspiration and hyperoxia. However, Mn SOD activity was unchanged. The decrease in Cu/Zn SOD was attenuated in animals, where serine proteinase activity was inhibited. However, antiproteinase treatment did not decrease acute pulmonary injury, as assessed by leakage of radiolabeled albumin into the lung (permeability index), arterial blood gases, and markers of acute inflammation (pulmonary myeloperoxidase activity, a surrogate neutrophilic marker, and inflammatory cytokine profiles). We conclude that production of serine proteinases play a major role in degrading Cu/Zn SOD, thereby decreasing pulmonary antioxidant capacity. However, the role this plays in the pathogenesis of the acute lung injury is not clear.

This article has been cited by other articles:

				M. D. McEvoy, S. T. Reeves, J. G. Reves, and F. G. Spinale Aprotinin in Cardiac Surgery: A Review of Conventional and Novel Mechanisms of Action Anesth. Analg., October 1, 2007; 105(4): 949 - 962. [Abstract] [Full Text] [PDF]

				B. H. Segal, B. A. Davidson, A. D. Hutson, T. A. Russo, B. A. Holm, B. Mullan, M. Habitzruther, S. M. Holland, and P. R. Knight III Acid aspiration-induced lung inflammation and injury are exacerbated in NADPH oxidase-deficient mice Am J Physiol Lung Cell Mol Physiol, March 1, 2007; 292(3): L760 - L768. [Abstract] [Full Text] [PDF]

Anesthesia & Analgesia® is published for the International Anesthesia Research Society® by Lippincott Williams & Wilkins with the assistance of Stanford University Libraries' HighWire Press®. Copyright 2006 by the International Anesthesia Research Society. Online ISSN: 1526-7598   Print ISSN: 0003-2999