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Anesth Analg 2005;101:1805-1808
© 2005 International Anesthesia Research Society
doi: 10.1213/01.ANE.0000184201.11804.4C

CRITICAL CARE AND TRAUMA

Drotrecogin Alfa (Activated) Does Not Affect Intracellular Production of Interleukin-6 and Tumor Necrosis Factor-{alpha} in Endotoxin-Stimulated Human Monocytes

Elke G. Czeslick, MD*, Frank Nestler*, Andreas Simm, PhD{dagger}, Anja Struppert*, and Armin Sablotzki, MD, PhD*

Departments of *Anesthesiology and Critical Care Medicine and {dagger}Cardiac and Thoracic Surgery, Martin Luther University Halle, Germany

Address correspondence and reprint requests to Elke Czeslick, MD, Department of Anesthesiology and Critical Care Medicine, Martin Luther University Halle, Ernst-Grube-Str. 40, 06120 Halle/Saale, Germany. Address e-mail to elke.czeslick{at}medizin.uni-halle.de.

In this ex vivo laboratory study, we investigated the effects of drotrecogin alfa (activated) (DA(a)), a recombinant form of human activated protein C, on the intracellular expression of interleukin (IL)-6 and tumor necrosis factor (TNF)-{alpha} on lipopolysaccharide (LPS)-stimulated human monocytes in a whole blood system. Whole blood samples from 10 healthy volunteers were stimulated with LPS (0.2 ng/mL) and incubated with 0.01, 0.1, 1, 10, and 100 nM of (final concentration) DA(a) for 3 h at 37°C and 5% CO2. Intracellular expression of IL-6 and TNF-{alpha} was assessed by flow cytometry. Our investigation showed that DA(a), at any of the concentrations tested, did not affect intracellular IL-6 and TNF-{alpha} production in LPS-stimulated human monocytes after 3 h of incubation. The results of this investigation led us to conclude that any antiinflammatory activity of DA(a), if present, does not occur via detectible decreases in the production of proinflammatory cytokines IL-6 and TNF-{alpha} in human monocytes.




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H. F. Galley, N. E. El Sakka, N. R. Webster, D. A. Lowes, and B. H. Cuthbertson
Activated protein C inhibits chemotaxis and interleukin-6 release by human neutrophils without affecting other neutrophil functions
Br. J. Anaesth., June 1, 2008; 100(6): 815 - 819.
[Abstract] [Full Text] [PDF]


Lippincott, Williams & Wilkins Anesthesia & Analgesia® is published for the International Anesthesia Research Society® by Lippincott Williams & Wilkins with the assistance of Stanford University Libraries' HighWire Press®. Copyright 2006 by the International Anesthesia Research Society. Online ISSN: 1526-7598   Print ISSN: 0003-2999 HighWire Press
Copyright © 2005 by the International Anesthesia Research Society.