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Anesth Analg 2006;103:620-625
© 2006 International Anesthesia Research Society
doi: 10.1213/01.ane.0000229714.09553.8c

ANESTHETIC PHARMACOLOGY

Transcutaneous Blood Gas CO2 Monitoring of Induced Ventilatory Depression in Mice

Peyman Sahbaie, MD*, Shohreh Madanlou, MD*, Parham Gharagozlou, MD*, J. David Clark, MD, PhD{dagger}, Jelveh Lameh, PhD*, and Timothy M. Delorey, PhD*

From the *Molecular Research Institute, Mountain View, California; {dagger}VA Palo Alto Health Care System and Stanford University Department of Anesthesiology, Palo Alto, California.

Address correspondence and reprint requests to Timothy M. Delorey, PhD, Molecular Research Institute, 2495 Old Middlefield Way, Mountain View, CA 94043. Address e-mail to tim{at}molres.org.

We assessed a simple, noninvasive method of monitoring transcutaneous partial pressure of CO2 (Ptcco2) in mice to determine whether it would provide an accurate and reproducible method to assess ventilatory depression in mice. To this end, Ptcco2 and Paco2 (partial pressure of arterial CO2) measurements were performed on isoflurane-anesthetized male C57Bl/6 mice breathing differing percentages of CO2 or fentanyl, a known ventilatory depressive drug. All doses of fentanyl produced a sharp increase in Ptcco2 values within 20 min with difference in Ptcco2 values between saline and all fentanyl groups being statistically significant (P < 0.0001). A good correlation between Paco2 and Ptcco2 values was established (r2 = 0.91). A Bland-Altman analysis likewise found that Ptcco2 measurements in the mice reliably and accurately reflected their Paco2 values. Therefore, under controlled conditions, Ptcco2 measurements were found to reliably reflect Paco2 values in mice. Consequently, the Ptcco2 method can be used as a means to rapidly and quantitatively assess the ventilatory depressive properties of a wide spectrum of drugs, under varying conditions in numerous mouse models.






Lippincott, Williams & Wilkins Anesthesia & Analgesia® is published for the International Anesthesia Research Society® by Lippincott Williams & Wilkins with the assistance of Stanford University Libraries' HighWire Press®. Copyright 2006 by the International Anesthesia Research Society. Online ISSN: 1526-7598   Print ISSN: 0003-2999 HighWire Press
Copyright © 2006 by the International Anesthesia Research Society.