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CARDIOVASCULAR ANESTHESIOLOGY

Differential Increase of Mitochondrial Matrix Volume by Sevoflurane in Isolated Cardiac Mitochondria

Matthias L. Riess, MD, PhD^*, Alexandre D. Costa, PhD, Richard Carlson, Jr, BS^*, Keith D. Garlid, MD, PhD, André Heinen, MD^*, and David F. Stowe, MD, PhD^||#

From the *Department of Anesthesiology, Medical College of Wisconsin, Milwaukee, Wisconsin; Department of Biology, Portland State University, Portland, Oregon; and Departments of Anesthesiology and Physiology, Cardiovascular Research Center, Medical College of Wisconsin and ||VA Medical Center Research Service, and #Department of Biomedical Engineering, Marquette University, Milwaukee, Wisconsin.

Address correspondence and reprint requests to Matthias L. Riess, MD, PhD, Medical College of Wisconsin, 8701 Watertown Plank Rd., Milwaukee, WI 53226. Address e-mail to mriess{at}mcw.edu.

Abstract

BACKGROUND: Mitochondrial (m) adenosine triphosphate sensitive potassium (K_ATP) channel opening has been reported to trigger and/or mediate cardioprotection by volatile anesthetics. However, the effects of volatile anesthetics on mitochondrial function are not well understood. Prevention of mitochondrial matrix volume (MMV) contraction during ischemia may contribute to cardioprotection against ischemia/reperfusion injury. We investigated whether sevoflurane increases MMV and if this increase is mediated by mK_ATP channel opening.

METHODS: Mitochondria from fresh guinea pig hearts were isolated and diluted in buffer that included oligomycin and ATP to inhibit ATP synthesis. Changes in MMV by diazoxide, a known mK_ATP channel opener, and by different sevoflurane concentrations, were measured by light absorption at 520 nm in the absence or presence of the mK_ATP channel blocker, 5-hydroxydecanoate.

RESULTS: Compared with control, 30–300 µM sevoflurane (approximately 0.2–2.1 vol %) increased MMV by 30%–55%, which was similar to the effect of diazoxide. These increases were blocked by 5-hydroxydecanoate. Higher sevoflurane concentration (1000 µM; 7.1 vol %), however, had no effect on MMV.

CONCLUSIONS: In clinically relevant concentrations, sevoflurane increases MMV via mK_ATP channel opening. Preservation of mitochondrial integrity may contribute to the cardioprotective effects of sevoflurane against ischemia/reperfusion injury. Impaired mitochondrial function at supraclinical anesthetic concentrations may explain the observed biphasic response. These findings add to our understanding of the intracellular mechanisms of volatile anesthetics as cardioprotective drugs.

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				A. D. T. Costa and K. D. Garlid Intramitochondrial signaling: interactions among mitoKATP, PKC{varepsilon}, ROS, and MPT Am J Physiol Heart Circ Physiol, August 1, 2008; 295(2): H874 - H882. [Abstract] [Full Text] [PDF]