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ANALGESIA

Use of Bulleyaconitine A as an Adjuvant for Prolonged Cutaneous Analgesia in the Rat

Chi-Fei Wang, MD^*, Peter Gerner, MD^*, Birgitta Schmidt, MD, Zhen Zhong Xu, PhD^*, Carla Nau, MD, Sho-Ya Wang, PhD, Ru-Rong Ji, PhD^*, and Ging Kuo Wang, PhD^*

From the Departments of *Anesthesiology, Perioperative and Pain Medicine, Pathology, Brigham and Women’s Hospital, Harvard Medical School, Boston, Massachusetts; Department of Anesthesiology, Friedrich-Alexander-University Erlangen-Nuremberg, Erlangen, Germany; and Department of Biology, State University of New York, Albany, New York.

Address correspondence and reprint requests to Ging Kuo Wang, PhD, Department of Anesthesiology, Perioperative and Pain Medicine, Brigham and Women’s Hospital, 75 Francis St., Boston, MA 02115. Address e-mail to wang{at}zeus.bwh.harvard.edu.

Abstract

BACKGROUND: Bulleyaconitine A (BLA) is an analgesic and antiinflammatory drug isolated from Aconitum plants. BLA has several potential targets, including voltage-gated Na⁺ channels. We tested whether BLA elicited long-lasting cutaneous analgesia, when co-injected with lidocaine and epinephrine, as a model for prolonged infiltration anesthesia.

METHODS: The local anesthetic properties of BLA were assessed by the patch-clamp technique in HEK293t cells expressing Nav1.7 and Nav1.8 neuronal Na⁺ channels, both crucial for nociception. Drug solutions (0.6 mL) were injected subcutaneously via rat shaved dorsal skin. Inhibition of the cutaneous trunci muscle reflex was evaluated by pinpricks. Skin cross-sections were stained with hematoxylin and eosin or with antibodies against PGP9.5.

RESULTS: BLA at 10 µM interacted minimally with resting or inactivated Nav1.7 and Nav1.8 Na⁺ channels when infrequently stimulated to +50 mV for 3 ms. However, when stimulated at 2 Hz for 1000 pulses, their peak Na⁺ currents were >90% reduced by BLA. This use-dependent inhibition was not significantly reversed after 15-min washing. Complete nociceptive blockade after injection of lidocaine (0.5%)/epinephrine (1:200,000) lasted for approximately 1 h in rats; full recovery occurred after approximately 6 h. Co-injection of 0.125 mM BLA with lidocaine/epinephrine increased the duration of complete nociceptive blockade to 24 h. Full recovery occurred after approximately 6 days. Skin histology including peripheral nerve fibers appeared unaffected by BLA.

CONCLUSIONS: BLA inhibits Nav1.7 and Nav1.8 Na⁺ currents in a use-dependent manner. Co-injection of BLA at 0.125 mM with lidocaine and epinephrine elicits complete cutaneous analgesia that lasts for up to 24 h without adverse effects.

Anesthesia & Analgesia® is published for the International Anesthesia Research Society® by Lippincott Williams & Wilkins with the assistance of Stanford University Libraries' HighWire Press®. Copyright 2006 by the International Anesthesia Research Society. Online ISSN: 1526-7598   Print ISSN: 0003-2999