JOURNAL HOME CME HOME THIS MONTH PAST ISSUES ETOC COLLECTIONS AUTHORS REVIEWERS EDITORIAL BOARD FEEDBACK RSS HELP
		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Email this article to a colleague Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Blanck, T. J. J. Articles by Thompson, M. Search for Related Content PubMed PubMed Citation Articles by Blanck, T. J. J. Articles by Thompson, M.

Calcium Uptake by Isolated Sarcoplasmic Rticulum

Examination of Halothane Inhibition, pH Dpendence, and Ca²⁺ Dependence of Normal and Malignant Hyperthermic Human Muscle

Thomas J. J. Blanck, MD, PhD^*, Raphael Gruener, PhD, Steven L. Suffecool, MD, and Meff Thompson, AB

*Assistant Professor of Anesthesiology, Department of Anesthsiology, University of Arizona, Health Sciences Center, Tescon, Arizona 85724. Professor of Physiology, Department of Physiology, University of Arizona, Health Sciences Center, Tescon, Arizona 85724. Instructor of Anesthesiology, Department of Anesthsiology, University of Arizona, Health Sciences Center, Tescon, Arizona 85724. Research Assistant, Department of Anesthsiology and Physiology, University of Arizona, Health Sciences Center, Tescon, Arizona 85724.

Abstract

Ca²⁺uptake and release in muscle homogenates and framented sarcoplasmic reticulum were examined in biopsy specimns from monsusceptible and malignant hyperthermia (MH) susceptible patients. Ca²⁺ flux was examind by the filter binding assy technique using⁴⁵ Ca. If was found that Ca²⁺ uptake and release were the same in both normal and MH muscle homogenates. Halothane inhibited the uptake of Ca²⁺ by the sarcoplasmic rticulum. The halothane inhibition of Ca²⁺ uptake in normal and MH sarcoplasmic reticulum was fitted to a single line with a correlation coefficint (r) of –0.958. The pH and Ca²⁺ dependence of Ca²⁺ dependence of Ca²⁺ uptake were the same for both normal and MH sarcoplasmic reticulum. The pK for Ca²⁺ uptake is approximately 5.9.It is concluded that the Ca²⁺ uptake function of the muscle from the five patients with MH examined is not abnormal and might not be the locus fr the initiation of MH.

Key Words: MUSCLE, Skeletal • HYPERTHERMIA: malignant • IONS: calcium • ANESTHETICS, Volatile: halothane

This article has been cited by other articles:

				D. A. Reuter, M. Anetseder, R. Muller, N. Roewer, and E. J. Hartung The ryanodine contracture test may help diagnose susceptiblity to malignant hyperthermia: [Le test de contracture a la ryanodine peut aider a decouvrir une susceptibilite a l'hyperthermie maligne] Can J Anesth, August 1, 2003; 50(7): 643 - 648. [Abstract] [Full Text] [PDF]

Anesthesia & Analgesia® is published for the International Anesthesia Research Society® by Lippincott Williams & Wilkins with the assistance of Stanford University Libraries' HighWire Press®. Copyright 2006 by the International Anesthesia Research Society. Online ISSN: 1526-7598   Print ISSN: 0003-2999