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CARDIOVASCULAR ANESTHESIA

Attenuation of Endothelium-Dependent Dilation of Pig Pulmonary Arterioles After Cardiopulmonary Bypass Is Prevented by Monoclonal Antibody to Complement C5a

Kyung W. Park, MD^*, Motohisa Tofukuji, MD, PhD, Caroline Metais, MD, Mark E. Comunale, MD^*, Hai B. Dai, MD, Michael Simons, MD, Gregory L. Stahl, PhD^§, Azin Agah, PhD^§, and Frank W. Sellke, MD

Departments of *Anesthesia and Critical Care, Surgery, and Medicine, Beth Israel Deaconess Medical Center and Center for Experimental Therapeutics and Reperfusion Injury; and §Department of Anesthesiology, Brigham & Women's Hospital, Harvard Medical School, Boston, Massachusetts

Address correspondence and reprint requests to Kyung W. Park, MD, Department of Anesthesia & Critical Care, 330 Brookline Ave., Boston, MA 02215. Address e-mail to kpark{at}caregroup.harvard.edu

We examined whether pulmonary endothelial dysfunction associated with cardiopulmonary bypass (CPB) may be mediated by complement C5a in pigs. Pigs were placed on normothermic CPB for 1 h with or without a previous administration of 1.6 mg/kg anti-C5a monoclonal antibody (MAb), then reperfused for 2 h. Pulmonary tissue myeloperoxidase activity was measured. Expression of nitric oxide synthase (NOS) was measured by reverse transcriptase polymerase chain reaction and Western blotting. Pulmonary arterioles approximately 100 µm in diameter were preconstricted with the thromboxane analog U46619 1 µM, and relaxation responses to adenosine diphosphate 10^-9–10^-4 M, substance P 10^-12–10^-6 M, and sodium nitroprusside 10^-9–10^-4 M were examined in vitro by videomicroscopy. Relaxation to the endothelium-dependent dilators adenosine diphosphate and substance P was attenuated after CPB; this attenuation was prevented by the previous administration of MAb. Relaxation to sodium nitroprusside was not affected by CPB. Neutrophil sequestration, as measured by MPO activity, increased after CPB, either with or without MAb. Transcription of NOS was unchanged by CPB, but translation of constitutive NOS was decreased after CPB, and this decrease was prevented by a previous administration of MAb. We conclude that pig pulmonary endothelial dysfunction associated with CPB may be mediated by C5a. The mechanism may involve changes in NOS translation.

Implications: In pigs, pulmonary endothelial dysfunction may occur after cardiopulmonary bypass due to product(s) of complement activation.

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Anesthesia & Analgesia® is published for the International Anesthesia Research Society® by Lippincott Williams & Wilkins with the assistance of Stanford University Libraries' HighWire Press®. Copyright 2006 by the International Anesthesia Research Society. Online ISSN: 1526-7598   Print ISSN: 0003-2999