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Anesth Analg 2001;93:424-429
© 2001 International Anesthesia Research Society

PAIN MEDICINE

The Residual Effects of Hemorrhagic Shock on Pain Reaction and C-Fos Expression in Rats

Taeko Fukuda, MD, Chikako Nishimoto, MD, Masayuki Miyabe, MD, and Hidenori Toyooka, MD

Department of Anesthesiology, Institute of Clinical Medicine, Tsukuba University, Tsukuba, Ibaraki, Japan

Address correspondence and reprint requests to Taeko Fukuda, MD, Department of Anesthesiology, Institute of Clinical Medicine, Tsukuba University, Tsukuba, Ibaraki, 305-8575, Japan. Address e-mail to e-mail: taekof{at}md.tsukuba.ac.jp

To investigate the residual effects of hemorrhagic shock on pain reaction and c-fos expression, we performed formalin tests after hemorrhage and reinfusion in rats. Twenty adult male Sprague-Dawley rats were divided into Control (n = 10) and Postshock (n = 10) groups. The mean blood pressure of the Control group was 100–120 mm Hg, and that of the Postshock group was kept at 50–60 mm Hg for 30 min by draining blood. After 15 min of returning mean blood pressure to normal levels in the Postshock group, 10% formalin (3.7% formaldehyde solution, 100 µL) was injected into the left rear paw of both groups. Nociceptive behaviors were observed for 1 h after the formalin injection. The rats were killed at 2 h after the formalin injection, and the lumbar spinal cord was then stained for c-fos immunohistochemistry by using the avidin-biotin-peroxidase method. Animals in the Postshock group showed considerably less nociceptive behavior than those in the Control group. C-fos expression in the deep layer (IV–VI) of the spinal cord was significantly less in the Postshock group. In conclusion, decreases of nociceptive behaviors and c-fos expression were observed under normotensive conditions after hemorrhagic shock. The mechanisms governing these reactions remain unclear.

IMPLICATIONS: Formalin tests were performed after hemorrhage and reinfusion in rats. A stress-induced analgesia was observed under normotensive conditions after hemorrhagic shock. The mechanisms remain unclear.






Lippincott, Williams & Wilkins Anesthesia & Analgesia® is published for the International Anesthesia Research Society® by Lippincott Williams & Wilkins with the assistance of Stanford University Libraries' HighWire Press®. Copyright 2006 by the International Anesthesia Research Society. Online ISSN: 1526-7598   Print ISSN: 0003-2999 HighWire Press
Copyright © 2001 by the International Anesthesia Research Society.