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ANESTHETIC PHARMACOLOGY

Tissue Antioxidant Capacity During Anesthesia: Propofol Enhances In Vivo Red Cell and Tissue Antioxidant Capacity in a Rat Model

Tim D. Runzer, MD^*, David M. Ansley, MD^*, David V. Godin, PhD, and Gordon K. Chambers, MD

Departments of *Anesthesia and Pharmacology and Therapeutics, University of British Columbia; and Department of Healthcare and Epidemiology, Center for Clinical Epidemiology and Evaluation, Vancouver Hospital and Health Sciences Centre, Vancouver, British Columbia, Canada

Address correspondence and reprint requests to David M. Ansley, MD, University of British Columbia Department of Anesthesia, Room 3200, 3rd floor, 910 West 10th Ave., Vancouver, BC, Canada V5Z 4E3. Address e-mail to daansley{at}interchange.ubc.ca

The effects of anesthesia on ischemia-reperfusion injury are of considerable scientific and clinical interest. We examined the effects of propofol (known to possess antioxidant activity) and halothane (devoid of antioxidant activity in vitro) on tissue and red blood cell (RBC) antioxidant capacity. Adult male Wistar rats were anesthetized with halothane 0.5%–1.0% (n = 7), propofol 500 µg · kg^-1 · min^-1 with halothane 0.25%–0.5% (small-dose propofol; n = 9), or propofol 2000 µg · kg^-1 · min^-1 (large-dose propofol; n = 8) for 45 min. Blood and tissue samples of liver, kidney, heart, and lung were then harvested for in vitro exposure to a peroxidizing agent. Red cell malondialdehyde and tissue thiobarbituric acid reactive substances were determined spectrophotometrically. Antioxidant capacities of blood and tissues in the Large-Dose Propofol group, and of blood and all tissues except lung in the Small-Dose Propofol group, were increased significantly compared with halothane (P < 0.003). The increases in tissue antioxidant capacities varied in their magnitude: RBC > liver > kidney > heart > lung. There was a high correlation between changes in RBC susceptibility to oxidative damage and corresponding changes in tissues. These findings demonstrate that large-dose propofol significantly enhances tissue antioxidant capacity, and RBC antioxidant capacity can serve as a functional measure of tissue activity, in vivo.

IMPLICATIONS: We designed this study to investigate the antioxidant effects of propofol in various tissues in a rat model. Pretreatment of animals with propofol led to a reduction in the susceptibility to an in vitro oxidative stress of five different tissues investigated, demonstrating the drug’s ability to limit oxidative injury. This may have future application in limiting organ dysfunction after periods of tissue ischemia (which results in oxidative damage).

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