JOURNAL HOME CME HOME THIS MONTH PAST ISSUES ETOC COLLECTIONS
AUTHORS REVIEWERS EDITORIAL BOARD FEEDBACK RSS HELP
A&A International Anesthesia Research Society
 QUICK SEARCH:   [advanced]


     


This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a colleague
Right arrow Similar articles in this journal
Right arrow Similar articles in ISI Web of Science
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Citing Articles
Right arrow Citing Articles via ISI Web of Science (4)
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Wang, S. Y.
Right arrow Articles by Segal, S.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Wang, S. Y.
Right arrow Articles by Segal, S.
Anesth Analg 2002;94:1304-1309
© 2002 International Anesthesia Research Society


OBSTETRIC ANESTHESIA

Pregnancy Alters Adrenergic Mechanisms in Uterine Arterioles of Rats

Steven Y. Wang, MD PhD, Sanjay Datta, MD, and Scott Segal, MD

Department of Anesthesiology, Perioperative, and Pain Medicine, Brigham and Women’s Hospital, Harvard Medical School, Boston, Massachusetts

Address correspondence and reprint requests to Steven Y. Wang, MD, PhD, c/o Scott Segal, MD, Department of Anesthesiology, Perioperative, and Pain Medicine, Brigham and Women’s Hospital, 75 Francis St., Boston, MA 02115. Address e-mail to swang{at}communitymedical.org

Pregnancy is associated with altered vascular reactivity. However, the effect of pregnancy on the {alpha}- and ß-adrenergic responses in the uterine microcirculation remains to be determined. In late-pregnant (Day 20–21, n = 6) and virgin (n = 6) Sprague-Dawley rats, uterine radial arterioles (70–120 µm in internal diameter) were isolated. We studied in vitro arteriolar responses in a pressurized, no-flow state with videomicroscopy. {alpha}2-Adrenergic activation relaxed uterine arterioles; this relaxation was increased with pregnancy and was inhibited after endothelial denudation or inhibition of nitric oxide synthase. Pregnancy significantly increased the contractile response to the {alpha}1-adrenoceptor agonist phenylephrine but decreased the relaxation to the ß-adrenoceptor agonist isoproterenol. The contractile response to the protein kinase C activator phorbol ester and relaxation responses to both the adenylate cyclase activator forskolin and the endothelium-independent cyclic guanosine monophosphate-mediated vaso- dilator nitroprusside were preserved. These results suggest that pregnancy enhances the {alpha}2-adrenoceptor-mediated relaxation of uterine arterioles, probably because of an increase in the release of nitric oxide. The {alpha}1-adrenergic response is upregulated, whereas the ß-adrenergic response is impaired, in the uterine microcirculation of pregnant rats.

IMPLICATIONS: Both {alpha}- and ß-adrenergic responses are important mechanisms for the regulation of uteroplacental perfusion. By use of an in vitromicrovascular technique, we have shown pregnancy-associated alteration in adrenergic responses in the uterine microcirculation of the rat.







Lippincott, Williams & Wilkins Anesthesia & Analgesia® is published for the International Anesthesia Research Society® by Lippincott Williams & Wilkins with the assistance of Stanford University Libraries' HighWire Press®. Copyright 2006 by the International Anesthesia Research Society. Online ISSN: 1526-7598   Print ISSN: 0003-2999 HighWire Press
Copyright © 2002 by the International Anesthesia Research Society.