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ANESTHETIC PHARMACOLOGY

The Effect of Repeated Isoflurane Anesthesia on Spatial and Psychomotor Performance in Young and Aged Mice

Noam N. Butterfield, PhD^*, Peter Graf, PhD, Craig R. Ries, MD FRCPC, PhD^*,, and Bernard A. MacLeod, MD FRCPC^*,

From the Centre for Anesthesia & Analgesia, Departments of *Pharmacology & Therapeutics, Psychology, and Anesthesia, The University of British Columbia, Vancouver, British Columbia

Address correspondence to Noam N. Butterfield, Centre for Anesthesia and Analgesia, Department of Pharmacology & Therapeutics, University of British Columbia, Vancouver, BC, Canada, V6T1Z3. Address email to noamb{at}canada.com

Exposure to general anesthesia may contribute to postoperative cognitive impairment in elderly patients, but the relationship remains poorly understood. We investigated whether aged mice, 18–19 mo, are more susceptible to postanesthetic cognitive impairment than young mice, 3–4 mo, using spatial memory (Barnes maze) and psychomotor (rotarod) tasks. Initially we studied the effect of a single anesthetic episode on asymptotic maze performance. We then tested whether repeated anesthesia would impair spatial memory and psychomotor performance to a greater extent in aged mice. Mice were anesthetized with isoflurane (1.4% atm) for 30 min; controls received 90% oxygen. Anesthesia, administered during the asymptotic period of maze learning, did not impair performance tested the following day (P > 0.05). Repeated anesthesia, 2–3 h after each session, did not impair overall maze or rotarod performance in young or aged mice (P > 0.05). Spatial learning appeared to be facilitated by anesthesia, F(1,204) = 7.97, P < 0.01 for pooled results. Asymptotic performance—when learning had stabilized—remained unimpaired in both the maze and rotarod tasks. These results suggest that an age-related risk of anesthetic-induced impairment appears to be limited to acquisition of a novel motor skill and that anesthesia alone does not lead to prolonged cognitive impairments in aged mice.

IMPLICATIONS: This study demonstrates that repeated isoflurane general anesthesia impaired psychomotor performance in aged mice during the initial learning period; however, spatial learning improved and, overall, spatial memory and psychomotor performance were unimpaired. Thus, general anesthesia alone does not appear to result in prolonged cognitive deficits in aged mice.

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